电离辐射通过转化生长因子-β-依赖性的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

内容可 ：探究远红外线是否可通过再生趋化因子-β（TGF-β）-介导的上皮-间质转换 (EMT)来增进细胞内的来袭迁到。应用于增幅2Gy(60)Coγ线紫外光由来进化器官的6种细胞内，历史纪录与EMT相关的变化，这包括分别借助于显微技术，细胞内印迹方法，免疫荧光技术，划痕试验和Transwell小室试验来掩蔽并样品细胞内组织形态，EMT记号，来袭迁到灵活性等。采用酶联免疫吸附法样品这些细胞内当中TGF-β蛋白程度，借助于引人注意诱发剂SB431542来评估TGF-β瞬时通路在远红外线EMT当中的作用。经过增幅为2Gy紫外光的细胞内当中不存在间叶细胞内的表达，与有假紫外光组相比其上皮记号减少，间叶细胞内记号增加，同时其来袭移往灵活性增强，TGF-β蛋白程度也减低。进一步见到由A549远红外线诱导的EMT可通过对TGF-β瞬时诱发发生逆转。这些结果表明TGF-β介导的EMT在远红外线诱导增强细胞内来袭移往灵活性当中起着关键作用。

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